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KMID : 0811720100140040205
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Korean Journal of Physiology & Pharmacology
2010 Volume.14 No. 4 p.205 ~ p.212
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Proteomic Analysis of Differentially Expressed Proteins in Bovine Endometrium with Endometritis
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Choe Chang-Yong
Kang Kee-Ryeon
Cho Myung-Je
Kim Eun-Suk
Park Jeong-Won
Park Sun-Young
Han Jae-Hee
Kang Da-Won
Lee Sung-Gyu
Lee Jeong-Soon
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Abstract
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Endometritis is one of the primary reasons for reproductive failure. In order to investigate endometritis-associated marker proteins, proteomic analysis was performed on bovine endometrium with endometritis. In bovine endometritis, desmin, ?-actin-2, heat-shock protein (HSP) 27, peroxiredoxin-6, luteinizing hormone receptor isoform 1, collectin-43 precursor, deoxyribonuclease-I (DNase-I), and MHC class I heavy chain (MHC-Ih) were up-regulated. In contrast, transferrin, interleukin-2 precursor, hemoglobin ? subunit, and potassium channel tetramerisation domain- containing 11 (KCTD11) were down-regulated in comparison to normal endometrium. The proteomic results were validated by semiquantitative-PCR and immunoblot analysis. The mRNA levels of desmin, transferrin, ?-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and ?-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and ?-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis.
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KEYWORD
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Actin, Desmin, Endometritis, Proteomics
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